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產品名稱：假單胞菌瓊脂基礎培養基（CN瓊脂）

Product name：CN Agar

產品用途：用于假單胞菌的選擇性分離培養。

Product usage: For selective isolation and culture of pseudomonas.

檢驗原理

Test principle

明膠蛋白胨和酸水解酪蛋白提供氮源；甘油提供碳源；硫酸鉀和氯化鎂可促進綠膿色素的產生；瓊脂是培養基的凝固劑；萘啶酮酸抑制非假單胞菌的革蘭氏陰性桿菌。

Gelatin peptone and acid hydrolyzed casein provide nitrogen sources. Glycerol provides carbon source; Potassium sulfate and magnesium chloride can promote the production of pyocyanin. AGAR is the coagulant of medium; Nalidixonic acid inhibits Gram-negative bacilli that are not pseudomonas.

配方成分

Formula ingredients

使用方法

Usage

秤取本品52.6g，加入蒸餾水1000ml，另加入10ml甘油，攪拌加熱煮沸至完全溶解，分裝，121℃高壓滅菌15分鐘，備用。

注：甘油需用戶自備。

注：培養基中含少量氯化鎂，滅菌后可能出現微量沉淀。

Weigh 52.6g of this product, add 1000ml of distilled water, add another 10ml of glycerol, stirring heating and boiling until completely dissolved, divided, 121 ℃ autoclave sterilization for 15 minutes, standby.

Note: Glycerol should be prepared by the user.

Note: The medium contains a small amount of magnesium chloride, trace precipitation may occur after sterilization.